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Luigi and The Three Sisters during the mini boss fight

The Old Graveyard is an area only accessible during the events of mission B-3 "Graveyard Shift", after Luigi goes through a secret passage from the tree root. Here there's a door leading to the Crypt, where a Toad Assistant can be found. After leaving that room, the three sisters The Three Sisters (Sister Lucina, Sister Herlinda and Sister Belinda) appear and attack Luigi, starting a mini boss battle.